Detailed information of TA system TA173528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 127466..128223
• Replicon: plasmid p1
• Accession: NZ_CP062205
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A136WTZ1
• Locus tag: IG172_RS25785
• Protein ID: WP_000501973.1
• Coordinates: 127466..127951 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: IG172_RS25790
• Protein ID: WP_001352814.1
• Coordinates: 127939..128223 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS25765	123197..124423	+	1227	WP_192292537.1	phosphoadenosine phosphosulfate reductase	-
IG172_RS25770	124408..125046	+	639	WP_069917554.1	ParB N-terminal domain-containing protein	-
IG172_RS25775	125210..125413	-	204	Protein_130	transposase	-
IG172_RS25780	126149..127171	+	1023	WP_069907201.1	IS110 family transposase	-
IG172_RS25785	127466..127951	-	486	WP_000501973.1	GNAT family N-acetyltransferase	Toxin
IG172_RS25790	127939..128223	-	285	WP_001352814.1	DUF1778 domain-containing protein	Antitoxin
IG172_RS25795	128614..130101	-	1488	WP_077888917.1	alpha/beta hydrolase	-
IG172_RS25800	130206..131153	-	948	WP_032215132.1	omptin family outer membrane protease OmpP	-
IG172_RS25805	131391..131546	-	156	Protein_136	transposase domain-containing protein	-
IG172_RS25810	131602..131944	-	343	Protein_137	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..144932	144932
	-	flank	IS/Tn	-	-	126149..127171	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-93)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17656.44 Da
• Isoelectric Point: 9.6133

>T173528 WP_000501973.1 NZ_CP062205:c127951-127466 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGETVLDDWLKQKGLKNQALGATRTFVVCRKGTQQVVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDASFRGKGLGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKHFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T173528 NZ_CP080235:4322360-4323280 [Escherichia coli O139:H1]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTTAAGCTGTTGATGGCTGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACTGATCTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATATAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10915.58 Da
• Isoelectric Point: 4.6926

>AT173528 WP_001352814.1 NZ_CP062205:c128223-127939 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT173528 NZ_CP080235:4321591-4322373 [Escherichia coli O139:H1]
TTGCCAAACTATCTAAACTGGTTGATGCAAAATACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAAAATGGCTGGCTCAGGCGCATCGCTCACGGCGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTGCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTACAGGGCCATGCGCACTATACCGAACCGGGCAGGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTAAAAATTGGCGACTGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A136WTZ1