Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 116285..116810 Replicon plasmid p1
Accession NZ_CP062205
Organism Escherichia coli strain C311

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IG172_RS25745 Protein ID WP_001159871.1
Coordinates 116285..116590 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag IG172_RS25750 Protein ID WP_000813630.1
Coordinates 116592..116810 (-) Length 73 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG172_RS25730 112234..113439 - 1206 WP_001401628.1 AAA family ATPase -
IG172_RS25735 114060..114791 + 732 WP_000504263.1 replication initiation protein -
IG172_RS25740 115478..116284 - 807 Protein_123 phage integrase family protein -
IG172_RS25745 116285..116590 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IG172_RS25750 116592..116810 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IG172_RS25755 119426..121639 + 2214 WP_077888293.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC 1..144932 144932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-100)

Antitoxin

(2-72)


Sequences


Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T173527 WP_001159871.1 NZ_CP062205:c116590-116285 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T173527 NZ_CP080235:4301118-4301516 [Escherichia coli O139:H1]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT173527 WP_000813630.1 NZ_CP062205:c116810-116592 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT173527 NZ_CP080235:4300822-4301115 [Escherichia coli O139:H1]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CCE8


Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References