Detailed information of TA system TA173519

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4549345..4550146
• Replicon: chromosome
• Accession: NZ_CP062204
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3W5RL53
• Locus tag: IG172_RS22120
• Protein ID: WP_041124173.1
• Coordinates: 4549769..4550146 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3W5RLB4
• Locus tag: IG172_RS22115
• Protein ID: WP_041124172.1
• Coordinates: 4549345..4549722 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS22080	4545246..4545926	+	681	WP_001282912.1	WYL domain-containing protein	-
IG172_RS22085	4546077..4546754	+	678	WP_021559490.1	hypothetical protein	-
IG172_RS22090	4546760..4546993	+	234	WP_001278287.1	DUF905 family protein	-
IG172_RS22095	4547092..4547910	+	819	WP_089599531.1	DUF945 domain-containing protein	-
IG172_RS22100	4548002..4548487	+	486	WP_041124170.1	antirestriction protein	-
IG172_RS22105	4548503..4548979	+	477	WP_021559500.1	RadC family protein	-
IG172_RS22110	4549048..4549269	+	222	WP_000692307.1	DUF987 domain-containing protein	-
IG172_RS22115	4549345..4549722	+	378	WP_041124172.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
IG172_RS22120	4549769..4550146	+	378	WP_041124173.1	TA system toxin CbtA family protein	Toxin
IG172_RS22125	4550143..4550631	+	489	WP_041124174.1	hypothetical protein	-
IG172_RS22130	4550643..4550840	+	198	WP_000839249.1	DUF957 domain-containing protein	-
IG172_RS22135	4550925..4551770	+	846	WP_053289820.1	DUF4942 domain-containing protein	-
IG172_RS22140	4551879..4552457	+	579	WP_053289821.1	hypothetical protein	-
IG172_RS22145	4552549..4552929	+	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
IG172_RS22150	4552926..4553273	+	348	WP_000612589.1	IS66 family insertion sequence element accessory protein TnpB	-
IG172_RS22155	4553323..4554861	+	1539	WP_000998048.1	IS66-like element ISEc8 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4526340..4550146	23806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13991.90 Da
• Isoelectric Point: 7.3225

>T173519 WP_041124173.1 NZ_CP062204:4549769-4550146 [Escherichia coli]
MNTLPDTHVRETSGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T173519 NZ_CP080235:c1316048-1315797 [Escherichia coli O139:H1]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13752.57 Da
• Isoelectric Point: 6.2021

>AT173519 WP_041124172.1 NZ_CP062204:4549345-4549722 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT173519 NZ_CP080235:c1315800-1315546 [Escherichia coli O139:H1]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A3W5RL53

Antitoxin

• AlphaFold DB: A0A3W5RLB4