Detailed information of TA system TA173517

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3841339..3842140
• Replicon: chromosome
• Accession: NZ_CP062204
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: IG172_RS18825
• Protein ID: WP_032215285.1
• Coordinates: 3841339..3841716 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IG172_RS18830
• Protein ID: WP_032215284.1
• Coordinates: 3841763..3842140 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS18795	3836597..3837553	+	957	WP_000121335.1	molybdenum cofactor insertion chaperone PaoD	-
IG172_RS18800	3837532..3837942	+	411	WP_001111349.1	transcriptional regulator	-
IG172_RS18805	3838280..3839599	+	1320	WP_000144688.1	site-specific integrase	-
IG172_RS18810	3839692..3840540	-	849	WP_137588141.1	DUF4942 domain-containing protein	-
IG172_RS18815	3840637..3840834	-	198	WP_000772024.1	DUF957 domain-containing protein	-
IG172_RS18820	3840854..3841342	-	489	WP_032215286.1	hypothetical protein	-
IG172_RS18825	3841339..3841716	-	378	WP_032215285.1	TA system toxin CbtA family protein	Toxin
IG172_RS18830	3841763..3842140	-	378	WP_032215284.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IG172_RS18835	3842303..3842524	-	222	WP_000692307.1	DUF987 domain-containing protein	-
IG172_RS18840	3842593..3843069	-	477	WP_001186774.1	RadC family protein	-
IG172_RS18845	3843085..3843564	-	480	WP_192291812.1	antirestriction protein	-
IG172_RS18850	3843664..3843864	+	201	WP_192292430.1	hypothetical protein	-
IG172_RS18855	3843903..3844724	-	822	WP_033811095.1	DUF945 domain-containing protein	-
IG172_RS18860	3845092..3845574	-	483	WP_077777324.1	hypothetical protein	-
IG172_RS18865	3845935..3846477	-	543	WP_033811096.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3812871..3873751	60880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14207.12 Da
• Isoelectric Point: 6.7029

>T173517 WP_032215285.1 NZ_CP062204:c3841716-3841339 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T173517 NZ_CP080235:c1118291-1118188 [Escherichia coli O139:H1]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13806.71 Da
• Isoelectric Point: 6.7387

>AT173517 WP_032215284.1 NZ_CP062204:c3842140-3841763 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT173517 NZ_CP080235:1118150-1118421 [Escherichia coli O139:H1]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT