Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3677276..3678113 Replicon chromosome
Accession NZ_CP062204
Organism Escherichia coli strain C311

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag IG172_RS18075 Protein ID WP_000227784.1
Coordinates 3677571..3678113 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag IG172_RS18070 Protein ID WP_001297137.1
Coordinates 3677276..3677587 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG172_RS18045 3672296..3673243 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
IG172_RS18050 3673265..3675256 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IG172_RS18055 3675246..3675860 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IG172_RS18060 3675860..3676189 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
IG172_RS18065 3676201..3677091 + 891 WP_000971336.1 heme o synthase -
IG172_RS18070 3677276..3677587 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
IG172_RS18075 3677571..3678113 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
IG172_RS18080 3678169..3679104 - 936 WP_001365761.1 sel1 repeat family protein -
IG172_RS18085 3679512..3680876 + 1365 WP_001000974.1 MFS transporter -
IG172_RS18090 3681004..3681495 - 492 WP_001138904.1 nucleotide binding protein YajQ -
IG172_RS18095 3681663..3682574 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T173516 WP_000227784.1 NZ_CP062204:3677571-3678113 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T173516 NZ_CP080235:c814624-814337 [Escherichia coli O139:H1]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT173516 WP_001297137.1 NZ_CP062204:3677276-3677587 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT173516 NZ_CP080235:c814863-814624 [Escherichia coli O139:H1]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References