Detailed information of TA system TA173504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1422943..1423568
• Replicon: chromosome
• Accession: NZ_CP062204
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IG172_RS07015
• Protein ID: WP_000911330.1
• Coordinates: 1423170..1423568 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IG172_RS07010
• Protein ID: WP_000450524.1
• Coordinates: 1422943..1423170 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS06985	1418746..1419216	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IG172_RS06990	1419216..1419788	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IG172_RS06995	1419934..1420812	+	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IG172_RS07000	1420829..1421863	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
IG172_RS07005	1422076..1422789	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IG172_RS07010	1422943..1423170	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IG172_RS07015	1423170..1423568	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IG172_RS07020	1423715..1424578	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
IG172_RS07025	1424593..1426608	+	2016	WP_000829342.1	tRNA cytosine(34) acetyltransferase TmcA	-
IG172_RS07030	1426682..1427380	+	699	WP_000679812.1	esterase	-
IG172_RS07035	1427500..1428480	-	981	WP_192292231.1	IS110-like element IS621 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1427500..1428480	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T173504 WP_000911330.1 NZ_CP062204:1423170-1423568 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T173504 NZ_CP080233:c3128647-3128393 [Escherichia coli O139:H1]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT173504 WP_000450524.1 NZ_CP062204:1422943-1423170 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT173504 NZ_CP080233:c3128895-3128644 [Escherichia coli O139:H1]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33