Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4518754..4519251 Replicon chromosome
Accession NZ_CP062203
Organism Escherichia coli strain K_EC180

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGX36_RS22060 Protein ID WP_001173343.1
Coordinates 4518754..4518927 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGX36_RS22065 Protein ID WP_000398619.1
Coordinates 4518955..4519251 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGX36_RS22050 4515424..4516881 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
IGX36_RS22055 4517118..4518635 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGX36_RS22060 4518754..4518927 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGX36_RS22065 4518955..4519251 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGX36_RS22070 4519479..4519751 - 273 WP_000405647.1 N-acetyltransferase -
IGX36_RS22075 4519763..4519993 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
IGX36_RS22080 4520173..4521804 + 1632 WP_001216482.1 sensor histidine kinase -
IGX36_RS22085 4521801..4522520 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4484026..4519251 35225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T173498 WP_001173343.1 NZ_CP062203:c4518927-4518754 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T173498 NZ_CP080233:c2210749-2210642 [Escherichia coli O139:H1]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTTGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT173498 WP_000398619.1 NZ_CP062203:c4519251-4518955 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT173498 NZ_CP080233:2210796-2210862 [Escherichia coli O139:H1]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References