Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4294783..4295359 Replicon chromosome
Accession NZ_CP062203
Organism Escherichia coli strain K_EC180

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IGX36_RS20935 Protein ID WP_001297643.1
Coordinates 4295072..4295359 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag IGX36_RS20930 Protein ID WP_000063160.1
Coordinates 4294783..4295085 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGX36_RS20915 4291379..4293529 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
IGX36_RS20920 4293579..4293782 + 204 WP_000467859.1 YbdD/YjiX family protein -
IGX36_RS20925 4293793..4294749 + 957 WP_001297640.1 GTPase -
IGX36_RS20930 4294783..4295085 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
IGX36_RS20935 4295072..4295359 - 288 WP_001297643.1 BrnT family toxin Toxin
IGX36_RS20940 4295558..4296255 - 698 Protein_4099 IS1 family transposase -
IGX36_RS20945 4296312..4296407 + 96 Protein_4100 dienelactone hydrolase family protein -
IGX36_RS20950 4296553..4297785 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
IGX36_RS20955 4297826..4299106 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T173497 WP_001297643.1 NZ_CP062203:c4295359-4295072 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T173497 NZ_CP080233:c2210214-2210107 [Escherichia coli O139:H1]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT173497 WP_000063160.1 NZ_CP062203:c4295085-4294783 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT173497 NZ_CP080233:2210262-2210328 [Escherichia coli O139:H1]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References