Detailed information of TA system TA173496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4158161..4158585
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: IGX36_RS20220
• Protein ID: WP_001306201.1
• Coordinates: 4158161..4158349 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: IGX36_RS20225
• Protein ID: WP_000125566.1
• Coordinates: 4158352..4158585 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS20190	4153723..4155009	+	1287	WP_000800470.1	peptidylprolyl isomerase SurA	-
IGX36_RS20195	4155009..4155998	+	990	WP_063086539.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
IGX36_RS20200	4155995..4156816	+	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
IGX36_RS20205	4156819..4157196	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
IGX36_RS20210	4157203..4158051	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
IGX36_RS20215	4158048..4158164	-	117	WP_000148634.1	CcdB family protein	-
IGX36_RS20220	4158161..4158349	-	189	WP_001306201.1	CcdB family protein	Toxin
IGX36_RS20225	4158352..4158585	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IGX36_RS20230	4158671..4159150	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
IGX36_RS20235	4159342..4161204	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
IGX36_RS20240	4161197..4161727	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
IGX36_RS20245	4161835..4163166	-	1332	WP_001183198.1	MFS transporter	-
IGX36_RS20250	4163225..4163512	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7167.44 Da
• Isoelectric Point: 11.6113

>T173496 WP_001306201.1 NZ_CP062203:c4158349-4158161 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T173496 NZ_CP080233:2038604-2038981 [Escherichia coli O139:H1]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATAACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT173496 WP_000125566.1 NZ_CP062203:c4158585-4158352 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT173496 NZ_CP080233:2038147-2038515 [Escherichia coli O139:H1]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9