Detailed information of TA system TA173490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2531074..2532662
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A3A6RNL0
• Locus tag: IGX36_RS12445
• Protein ID: WP_063085289.1
• Coordinates: 2531340..2532662 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IGX36_RS12440
• Protein ID: WP_001296726.1
• Coordinates: 2531074..2531340 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS12420	2526074..2527049	+	976	Protein_2431	hypothetical protein	-
IGX36_RS12425	2527046..2527255	+	210	Protein_2432	transporter	-
IGX36_RS12430	2527256..2529352	+	2097	Protein_2433	ATPase	-
IGX36_RS12435	2529391..2530203	+	813	Protein_2434	autotransporter outer membrane beta-barrel domain-containing protein	-
IGX36_RS12440	2531074..2531340	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IGX36_RS12445	2531340..2532662	+	1323	WP_063085289.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IGX36_RS12450	2533059..2533956	-	898	Protein_2437	LysR family transcriptional regulator	-
IGX36_RS12455	2534096..2534404	+	309	WP_001221046.1	hypothetical protein	-
IGX36_RS12460	2534413..2535102	+	690	WP_000801314.1	type 1 glutamine amidotransferase domain-containing protein	-
IGX36_RS12465	2535164..2536111	+	948	WP_063085290.1	aldo/keto reductase	-
IGX36_RS12470	2536214..2536369	+	156	WP_001327998.1	hypothetical protein	-
IGX36_RS12475	2536329..2536748	+	420	WP_001314733.1	amidohydrolase family protein	-
IGX36_RS12480	2536699..2536890	+	192	WP_001306054.1	amidohydrolase family protein	-
IGX36_RS12485	2537040..2537612	+	573	WP_000789914.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2526765..2554928	28163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49313.91 Da
• Isoelectric Point: 8.5849

>T173490 WP_063085289.1 NZ_CP062203:2531340-2532662 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDHVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T173490 NZ_CP080233:c509554-509177 [Escherichia coli O139:H1]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTTACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTTAC
CAGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACGCCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATATCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACAAGCCGGGATTC
AACGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTGAACAATATCACCCGGGGTAAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT173490 WP_001296726.1 NZ_CP062203:2531074-2531340 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT173490 NZ_CP080233:c510012-509644 [Escherichia coli O139:H1]
GTGTCAGACACACTCCATGAAACAAATTATCCCGACGACAACAACGACCGCTCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCAGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGTCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A3A6RNL0