Detailed information of TA system TA173489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2469676..2470202
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IGX36_RS12090
• Protein ID: WP_000323025.1
• Coordinates: 2469915..2470202 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IGX36_RS12085
• Protein ID: WP_000534858.1
• Coordinates: 2469676..2469915 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS12050	2464694..2464879	+	186	Protein_2357	hypothetical protein	-
IGX36_RS12055	2464877..2465017	+	141	Protein_2358	DUF977 family protein	-
IGX36_RS12060	2465014..2465369	+	356	Protein_2359	class I SAM-dependent methyltransferase	-
IGX36_RS12065	2465980..2466267	+	288	Protein_2360	hypothetical protein	-
IGX36_RS12070	2467036..2468349	+	1314	WP_000589005.1	ISNCY family transposase	-
IGX36_RS12075	2468811..2469143	-	333	WP_001301033.1	protein FlxA	-
IGX36_RS12080	2469346..2469651	-	306	WP_001326990.1	hypothetical protein	-
IGX36_RS12085	2469676..2469915	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IGX36_RS12090	2469915..2470202	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IGX36_RS12095	2470274..2470429	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
IGX36_RS12100	2470646..2470897	+	252	WP_000980994.1	hypothetical protein	-
IGX36_RS12105	2470964..2471242	+	279	WP_012304870.1	hypothetical protein	-
IGX36_RS12110	2471244..2472293	+	1050	WP_063085032.1	DUF968 domain-containing protein	-
IGX36_RS12115	2472307..2473059	+	753	WP_001047135.1	antitermination protein	-
IGX36_RS12120	2473337..2473426	-	90	WP_120795389.1	hypothetical protein	-
IGX36_RS12125	2473481..2473693	-	213	WP_000087756.1	cold shock-like protein CspF	-
IGX36_RS12130	2473994..2474209	+	216	WP_000066484.1	cold shock-like protein CspB	-
IGX36_RS12135	2474963..2475178	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2458012..2489535	31523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T173489 WP_000323025.1 NZ_CP062203:2469915-2470202 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T173489 NZ_CP080233:497780-497953 [Escherichia coli O139:H1]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT173489 WP_000534858.1 NZ_CP062203:2469676-2469915 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT173489 NZ_CP080233:497456-497752 [Escherichia coli O139:H1]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8