Detailed information of TA system TA173478

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 827154..827989
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: IGX36_RS03895
• Protein ID: WP_000854821.1
• Coordinates: 827154..827531 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: IGX36_RS03900
• Protein ID: WP_001285610.1
• Coordinates: 827621..827989 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS03865	822281..823429	-	1149	WP_000905931.1	capsule polysaccharide export inner-membrane protein KpsE	-
IGX36_RS03870	823501..824484	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
IGX36_RS03875	825295..825465	-	171	Protein_759	IS110 family transposase	-
IGX36_RS03880	825808..826650	-	843	WP_001280493.1	DUF4942 domain-containing protein	-
IGX36_RS03885	826735..826932	-	198	WP_085949090.1	DUF957 domain-containing protein	-
IGX36_RS03890	826960..827157	-	198	Protein_762	hypothetical protein	-
IGX36_RS03895	827154..827531	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
IGX36_RS03900	827621..827989	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IGX36_RS03905	828069..828290	-	222	WP_000692350.1	DUF987 domain-containing protein	-
IGX36_RS03910	828570..828709	+	140	Protein_766	hypothetical protein	-
IGX36_RS03915	829429..829998	+	570	WP_000220729.1	inovirus Gp2 family protein	-
IGX36_RS03920	830164..830547	+	384	WP_000271026.1	hypothetical protein	-
IGX36_RS03925	830561..831757	-	1197	WP_001372615.1	IS110 family transposase	-
IGX36_RS03930	832318..832875	+	558	WP_000736772.1	Ail/Lom family outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	830561..831757	1196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T173478 WP_000854821.1 NZ_CP062203:c827531-827154 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T173478 NZ_CP080223:c4489067-4488894 [Escherichia coli O141:H4]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT173478 WP_001285610.1 NZ_CP062203:c827989-827621 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT173478 NZ_CP080223:c4489391-4489095 [Escherichia coli O141:H4]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7W4PV54