Detailed information of TA system TA173476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 1589869..1590668
• Replicon: chromosome
• Accession: NZ_CP062196
• Organism: Plesiomonas shigelloides strain P5462

Toxin (Protein)

• Gene name: -
• Uniprot ID: R8AQZ4
• Locus tag: IHE26_RS06905
• Protein ID: WP_010863418.1
• Coordinates: 1590135..1590668 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8AQZ3
• Locus tag: IHE26_RS06900
• Protein ID: WP_010863417.1
• Coordinates: 1589869..1590138 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHE26_RS06890	1586449..1588307	+	1859	Protein_1318	UvrD-helicase domain-containing protein	-
IHE26_RS06895	1588889..1589578	+	690	WP_192437683.1	methyltransferase domain-containing protein	-
IHE26_RS06900	1589869..1590138	+	270	WP_010863417.1	DUF1778 domain-containing protein	Antitoxin
IHE26_RS06905	1590135..1590668	+	534	WP_010863418.1	GNAT family N-acetyltransferase	Toxin
IHE26_RS06910	1590886..1591434	-	549	WP_192437685.1	hypothetical protein	-
IHE26_RS06915	1592565..1592804	-	240	WP_084978288.1	hypothetical protein	-
IHE26_RS06920	1593204..1593839	-	636	WP_192437686.1	NADPH-dependent F420 reductase	-
IHE26_RS06925	1594160..1594576	-	417	WP_192437688.1	hypothetical protein	-
IHE26_RS06930	1594984..1595121	-	138	WP_192437690.1	DUF3265 domain-containing protein	-
IHE26_RS06935	1595112..1595495	-	384	WP_192437692.1	DUF1311 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	1541277..1622172	80895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (95-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-81)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20266.68 Da
• Isoelectric Point: 8.8293

>T173476 WP_010863418.1 NZ_CP062196:1590135-1590668 [Plesiomonas shigelloides]
VSWSDKFVELDKSKHDRESFYCGESELDTFIQKFALKHMSAGISRTMVLPAKPVLPNNKLPISAFYTIAPSSICRDTLPE
LLAKKLPRYPIPVFLLAQLSVHSMYQGCGLGKVALIKALQYLWQVNSYIRAYAVVVDCLNENAESFYLKYGFEYLCEHNG
RKRLFLPMKTIEQLFNS

Nucleotide

• Length: 534 bp

>T173476 NZ_CP080223:3858417-3858695 [Escherichia coli O141:H4]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9882.34 Da
• Isoelectric Point: 4.9059

>AT173476 WP_010863417.1 NZ_CP062196:1589869-1590138 [Plesiomonas shigelloides]
MATARLDLRIDEEIKAKAEKASALLGLKSLTEYVVRLMDENSTKVIAEHESITVSNDIFDQFIEACDKAKAPNEALKDAL
AFTKEKGIR

Nucleotide

• Length: 270 bp

>AT173476 NZ_CP080223:3858154-3858414 [Escherichia coli O141:H4]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: R8AQZ4

Antitoxin

• AlphaFold DB: R8AQZ3