Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5409192..5409823 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag IEM32_RS26800 Protein ID WP_001259386.1
Coordinates 5409548..5409823 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IEM32_RS26795 Protein ID WP_000593555.1
Coordinates 5409192..5409551 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS26765 5404304..5404777 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
IEM32_RS26770 5404774..5405055 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
IEM32_RS26775 5405132..5406490 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
IEM32_RS26780 5406483..5407991 + 1509 WP_001301773.1 carbohydrate kinase -
IEM32_RS26785 5407981..5408250 + 270 WP_000933060.1 HPr family phosphocarrier protein -
IEM32_RS26790 5408282..5409142 + 861 WP_001131788.1 class II aldolase -
IEM32_RS26795 5409192..5409551 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IEM32_RS26800 5409548..5409823 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
IEM32_RS26805 5409896..5411020 - 1125 WP_001301659.1 ABC transporter permease -
IEM32_RS26810 5411020..5413755 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
IEM32_RS26815 5413752..5414819 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T173462 WP_001259386.1 NZ_CP062160:c5409823-5409548 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T173462 NZ_CP080223:625576-625890 [Escherichia coli O141:H4]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT173462 WP_000593555.1 NZ_CP062160:c5409551-5409192 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT173462 NZ_CP080223:625887-626303 [Escherichia coli O141:H4]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References