Detailed information of TA system TA173460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 5341252..5343580
• Replicon: chromosome
• Accession: NZ_CP062160
• Organism: Escherichia coli strain 20R2R

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: IEM32_RS26430
• Protein ID: WP_000532146.1
• Coordinates: 5342606..5343580 (-)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: IEM32_RS26425
• Protein ID: WP_001303704.1
• Coordinates: 5341252..5342547 (-)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEM32_RS26410	5336859..5339045	-	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-
IEM32_RS26415	5339318..5340421	-	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
IEM32_RS26420	5340613..5341206	+	594	WP_001002544.1	NAAT family transporter YhgN	-
IEM32_RS26425	5341252..5342547	-	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
IEM32_RS26430	5342606..5343580	-	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
IEM32_RS26435	5343584..5343985	-	402	WP_000502498.1	ATP-dependent DNA helicase	-
IEM32_RS26440	5343970..5344578	-	609	WP_000410808.1	ATP-dependent DNA helicase	-
IEM32_RS26445	5344718..5346058	-	1341	WP_000210111.1	gluconate transporter	-
IEM32_RS26450	5346062..5346589	-	528	WP_000108330.1	gluconokinase	-
IEM32_RS26455	5346728..5347723	-	996	WP_071338730.1	gluconate operon transcriptional repressor GntR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T173460 WP_000532146.1 NZ_CP062160:c5343580-5342606 [Escherichia coli]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T173460 NZ_CP080223:168704-168811 [Escherichia coli O141:H4]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT173460 WP_001303704.1 NZ_CP062160:c5342547-5341252 [Escherichia coli]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT173460 NZ_CP080223:c168656-168590 [Escherichia coli O141:H4]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8