Detailed information of TA system TA173459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 5317708..5318462
• Replicon: chromosome
• Accession: NZ_CP062160
• Organism: Escherichia coli strain 20R2R

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: IEM32_RS26335
• Protein ID: WP_001301452.1
• Coordinates: 5317977..5318462 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: IEM32_RS26330
• Protein ID: WP_000801912.1
• Coordinates: 5317708..5317986 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEM32_RS26325	5314936..5317641	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
IEM32_RS26330	5317708..5317986	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
IEM32_RS26335	5317977..5318462	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
IEM32_RS26340	5318512..5319540	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
IEM32_RS26345	5319544..5320770	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
IEM32_RS26350	5320958..5322556	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
IEM32_RS26355	5322538..5323296	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T173459 WP_001301452.1 NZ_CP062160:5317977-5318462 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T173459 NZ_CP080222:c2569710-2569444 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT173459 WP_000801912.1 NZ_CP062160:5317708-5317986 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT173459 NZ_CP080222:c2569967-2569710 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9