Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2971480..2972118 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IEM32_RS14375 Protein ID WP_000813794.1
Coordinates 2971480..2971656 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IEM32_RS14380 Protein ID WP_001270286.1
Coordinates 2971702..2972118 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS14355 2967102..2968313 - 1212 WP_071525382.1 BenE family transporter YdcO -
IEM32_RS14360 2968366..2968902 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
IEM32_RS14365 2968975..2970936 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IEM32_RS14370 2971028..2971258 - 231 WP_000494244.1 YncJ family protein -
IEM32_RS14375 2971480..2971656 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IEM32_RS14380 2971702..2972118 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IEM32_RS14385 2972197..2973603 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
IEM32_RS14390 2973848..2974993 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
IEM32_RS14395 2975011..2976024 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
IEM32_RS14400 2976025..2976966 + 942 WP_001251320.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T173453 WP_000813794.1 NZ_CP062160:2971480-2971656 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T173453 NZ_CP080222:c882296-882108 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT173453 WP_001270286.1 NZ_CP062160:2971702-2972118 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT173453 NZ_CP080222:c882585-882325 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTATATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References