Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1629226..1629844 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IEM32_RS07695 Protein ID WP_001291435.1
Coordinates 1629226..1629444 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IEM32_RS07700 Protein ID WP_000344800.1
Coordinates 1629470..1629844 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS07660 1624513..1625085 + 573 WP_000779831.1 YbaY family lipoprotein -
IEM32_RS07665 1625118..1625429 - 312 WP_000409911.1 MGMT family protein -
IEM32_RS07675 1625808..1626161 + 354 WP_000878140.1 DUF1428 domain-containing protein -
IEM32_RS07680 1626203..1627753 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IEM32_RS07685 1627917..1628387 - 471 WP_000136192.1 YlaC family protein -
IEM32_RS07690 1628503..1629054 - 552 WP_000102553.1 maltose O-acetyltransferase -
IEM32_RS07695 1629226..1629444 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IEM32_RS07700 1629470..1629844 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IEM32_RS07705 1630390..1633539 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
IEM32_RS07710 1633562..1634755 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173437 WP_001291435.1 NZ_CP062160:c1629444-1629226 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T173437 NZ_CP080172:c3589859-3589452 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT173437 WP_000344800.1 NZ_CP062160:c1629844-1629470 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT173437 NZ_CP080172:c3590106-3589840 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References