Detailed information of TA system TA173434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1137860..1138410
• Replicon: chromosome
• Accession: NZ_CP062160
• Organism: Escherichia coli strain 20R2R

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: IEM32_RS05395
• Protein ID: WP_001160975.1
• Coordinates: 1138096..1138410 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: IEM32_RS05390
• Protein ID: WP_000125566.1
• Coordinates: 1137860..1138093 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEM32_RS05365	1132934..1133221	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
IEM32_RS05370	1133279..1134610	+	1332	WP_001183177.1	MFS transporter	-
IEM32_RS05375	1134718..1135248	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
IEM32_RS05380	1135241..1137103	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
IEM32_RS05385	1137295..1137774	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
IEM32_RS05390	1137860..1138093	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IEM32_RS05395	1138096..1138410	+	315	WP_001160975.1	CcdB family protein	Toxin
IEM32_RS05400	1138407..1139255	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
IEM32_RS05405	1139262..1139639	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
IEM32_RS05410	1139642..1140463	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
IEM32_RS05415	1140460..1141449	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
IEM32_RS05420	1141449..1142735	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T173434 WP_001160975.1 NZ_CP062160:1138096-1138410 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T173434 NZ_CP080172:c2662718-2662467 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT173434 WP_000125566.1 NZ_CP062160:1137860-1138093 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT173434 NZ_CP080172:c2662470-2662216 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACTGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9