Detailed information of TA system TA173433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 888545..889140
• Replicon: chromosome
• Accession: NZ_CP062160
• Organism: Escherichia coli strain 20R2R

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: IEM32_RS04345
• Protein ID: WP_000239579.1
• Coordinates: 888790..889140 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: IEM32_RS04340
• Protein ID: WP_001223210.1
• Coordinates: 888545..888796 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEM32_RS04330	884210..887989	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
IEM32_RS04335	887992..888333	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
IEM32_RS04340	888545..888796	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
IEM32_RS04345	888790..889140	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
IEM32_RS04350	889220..889750	-	531	WP_000055075.1	inorganic diphosphatase	-
IEM32_RS04355	890060..891016	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
IEM32_RS04360	891156..892658	+	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
IEM32_RS04365	892672..893694	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T173433 WP_000239579.1 NZ_CP062160:888790-889140 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T173433 NZ_CP080172:c2662470-2662216 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACTGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT173433 WP_001223210.1 NZ_CP062160:888545-888796 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT173433 NZ_CP080172:c2662718-2662467 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8