Detailed information of TA system TA173421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3256707..3257279
• Replicon: chromosome
• Accession: NZ_CP062147
• Organism: Komagataeibacter hansenii strain C110

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IFJ82_RS14185
• Protein ID: WP_003618720.1
• Coordinates: 3256707..3257036 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HXX4
• Locus tag: IFJ82_RS14190
• Protein ID: WP_003618721.1
• Coordinates: 3257037..3257279 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ82_RS14160	3252552..3252812	+	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
IFJ82_RS14165	3252809..3253102	+	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
IFJ82_RS14170	3253233..3254495	+	1263	WP_003618713.1	sodium:proton antiporter	-
IFJ82_RS14175	3254495..3255178	+	684	WP_003618716.1	DUF1275 domain-containing protein	-
IFJ82_RS14180	3255433..3256506	+	1074	WP_003618718.1	alkene reductase	-
IFJ82_RS14185	3256707..3257036	-	330	WP_003618720.1	endoribonuclease MazF	Toxin
IFJ82_RS14190	3257037..3257279	-	243	WP_003618721.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IFJ82_RS14195	3257819..3259498	-	1680	Protein_2776	conjugal transfer protein TraG	-
IFJ82_RS14200	3259618..3260037	+	420	WP_003618779.1	aromatic ring-cleaving dioxygenase	-
IFJ82_RS14205	3260142..3260768	+	627	WP_003618778.1	hypothetical protein	-
IFJ82_RS14210	3260773..3261363	+	591	WP_003618777.1	RES family NAD+ phosphorylase	-
IFJ82_RS14215	3261540..3261938	-	399	WP_080937632.1	hypothetical protein	-
IFJ82_RS14220	3261972..3262250	-	279	WP_010669065.1	DUF2285 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12221.05 Da
• Isoelectric Point: 9.2806

>T173421 WP_003618720.1 NZ_CP062147:c3257036-3256707 [Komagataeibacter hansenii]
MSAWVPDCGDIVWLEFDPQAGREQSGHRPAVVLSPVSYNKKSGMIVCCPTTTKIKGYPFEVPLTGEPTSVVLSDQVRSLD
WRARRAKHKGRVSERELEAVRERTRLLVG

Nucleotide

• Length: 330 bp

>T173421 NZ_CP080172:191003-191389 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8730.86 Da
• Isoelectric Point: 4.9810

>AT173421 WP_003618721.1 NZ_CP062147:c3257279-3257037 [Komagataeibacter hansenii]
MHGTVRKWGNSAAIRLPANILEAVHLQINQAVDVREEDGRIIIQPVHPAVLSLDTLTAAITDDNRHGEVSFGPEIGGEIW

Nucleotide

• Length: 243 bp

>AT173421 NZ_CP080172:190464-190973 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T672