Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 19433..20169 Replicon plasmid pC
Accession NZ_CP062141
Organism Klebsiella quasipneumoniae strain 203 19001

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag IG177_RS26560 Protein ID WP_004098919.1
Coordinates 19433..19915 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag IG177_RS26565 Protein ID WP_004098921.1
Coordinates 19903..20169 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG177_RS26545 16221..16931 - 711 WP_127114021.1 PilZ domain-containing protein -
IG177_RS26550 17189..18157 - 969 WP_087728122.1 IS5 family transposase -
IG177_RS26555 18188..19237 - 1050 Protein_17 ISNCY family transposase -
IG177_RS26560 19433..19915 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
IG177_RS26565 19903..20169 - 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
IG177_RS26570 20443..20706 + 264 WP_064798521.1 hypothetical protein -
IG177_RS26575 20707..21057 + 351 WP_064798520.1 hypothetical protein -
IG177_RS26580 21247..21741 - 495 WP_064798519.1 hypothetical protein -
IG177_RS26585 21772..22344 - 573 WP_064798518.1 hypothetical protein -
IG177_RS26590 22341..22589 - 249 WP_064798517.1 hypothetical protein -
IG177_RS26595 22879..23457 + 579 WP_064798526.1 hypothetical protein -
IG177_RS26600 23596..24597 - 1002 WP_032635019.1 Abi family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..81122 81122


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T173408 WP_004098919.1 NZ_CP062141:c19915-19433 [Klebsiella quasipneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T173408 NZ_CP080163:3628486-3628980 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT173408 WP_004098921.1 NZ_CP062141:c20169-19903 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT173408 NZ_CP080163:3628196-3628489 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References