Detailed information of TA system TA173394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 38836..39413
• Replicon: plasmid pr9-0915
• Accession: NZ_CP062125
• Organism: Listeria monocytogenes strain FSL R9-0915

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: IFI79_RS14800
• Protein ID: WP_003728465.1
• Coordinates: 38836..39177 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: IFI79_RS14805
• Protein ID: WP_003728464.1
• Coordinates: 39171..39413 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFI79_RS14785	35315..35869	-	555	WP_003728468.1	recombinase family protein	-
IFI79_RS14790	36149..36508	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
IFI79_RS14795	36508..38643	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
IFI79_RS14800	38836..39177	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IFI79_RS14805	39171..39413	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IFI79_RS14810	39474..39725	-	252	WP_003728463.1	hypothetical protein	-
IFI79_RS14815	39856..40182	+	327	WP_003728462.1	hypothetical protein	-
IFI79_RS14820	40182..40844	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
IFI79_RS14825	40863..41240	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
IFI79_RS14830	41399..41887	+	489	WP_003728520.1	hypothetical protein	-
IFI79_RS14835	42009..42257	+	249	WP_003728519.1	hypothetical protein	-
IFI79_RS14840	42258..42683	+	426	WP_003728518.1	hypothetical protein	-
IFI79_RS14845	42687..43409	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
IFI79_RS14850	43425..44141	+	717	WP_003731678.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50101	50101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T173394 WP_003728465.1 NZ_CP062125:c39177-38836 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T173394 NZ_CP080163:c138073-137900 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT173394 WP_003728464.1 NZ_CP062125:c39413-39171 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT173394 NZ_CP080163:c138397-138101 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5