Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 5425088..5425677 Replicon chromosome
Accession NZ_CP062122
Organism Pseudomonas sp. ADPe

Toxin (Protein)


Gene name pemK Uniprot ID A0A1A5D4S0
Locus tag IG194_RS24750 Protein ID WP_058489027.1
Coordinates 5425088..5425444 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID W5IWC6
Locus tag IG194_RS24755 Protein ID WP_009618989.1
Coordinates 5425438..5425677 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG194_RS24725 5420563..5421657 + 1095 WP_058070121.1 D-alanine--D-alanine ligase -
IG194_RS24730 5421679..5421906 - 228 WP_141679779.1 hypothetical protein -
IG194_RS24735 5422453..5422881 + 429 WP_134163460.1 hypothetical protein -
IG194_RS24740 5423119..5423712 + 594 WP_058070123.1 histidine phosphatase family protein -
IG194_RS24745 5423804..5424613 + 810 WP_058489026.1 class I SAM-dependent methyltransferase -
IG194_RS24750 5425088..5425444 - 357 WP_058489027.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IG194_RS24755 5425438..5425677 - 240 WP_009618989.1 antitoxin PemI Antitoxin
IG194_RS24760 5425808..5426413 - 606 WP_043315086.1 LysE family translocator -
IG194_RS24765 5426465..5427301 - 837 WP_058489028.1 AraC family transcriptional regulator -
IG194_RS24770 5427384..5427881 - 498 WP_058489029.1 MarR family transcriptional regulator -
IG194_RS24775 5427999..5429159 + 1161 WP_058489030.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(11-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13132.99 Da        Isoelectric Point: 7.9176

>T173393 WP_058489027.1 NZ_CP062122:c5425444-5425088 [Pseudomonas sp. ADPe]
MVRRPPSVPNRGEIWHLQFDPSTGREMIGDHFCLVLSKREFNEMGLALVCPISQGASNVARNQGFLVTLMGEGTDTQGNV
HSHQVRTLDWRARRAAFKEKAPDSVTNYVLSLVGAILE

Download         Length: 357 bp

>T173393 NZ_CP080163:125484-125861 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8345.65 Da        Isoelectric Point: 4.8668

>AT173393 WP_009618989.1 NZ_CP062122:c5425677-5425438 [Pseudomonas sp. ADPe]
MTTLALRKSGGSLIISVPSAFVAQNKLHAGSQVECDIVEGALVIKPMTRPSLAQLIAETPKDSLAPDWDSMPAVGAEQW

Download         Length: 240 bp

>AT173393 NZ_CP080163:125063-125437 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A5D4S0

Antitoxin

Source ID Structure
AlphaFold DB A0A1A5D3H0

References