Detailed information of TA system TA173391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4675263..4675871
• Replicon: chromosome
• Accession: NZ_CP062122
• Organism: Pseudomonas sp. ADPe

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A1A5DDR6
• Locus tag: IG194_RS21255
• Protein ID: WP_058488662.1
• Coordinates: 4675263..4675610 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: IG194_RS21260
• Protein ID: WP_081884705.1
• Coordinates: 4675620..4675871 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG194_RS21240	4672021..4672695	-	675	WP_058488660.1	DUF4194 domain-containing protein	-
IG194_RS21245	4672727..4674199	-	1473	WP_009618251.1	DUF3375 domain-containing protein	-
IG194_RS21250	4674295..4674900	-	606	WP_058488661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
IG194_RS21255	4675263..4675610	-	348	WP_058488662.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IG194_RS21260	4675620..4675871	-	252	WP_081884705.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IG194_RS21265	4676328..4677743	+	1416	WP_058488663.1	amino acid permease	-
IG194_RS21270	4677869..4678405	+	537	WP_058488664.1	2,4'-dihydroxyacetophenone dioxygenase family protein	-
IG194_RS21275	4678462..4679367	+	906	WP_058488665.1	LysR family transcriptional regulator	-
IG194_RS21280	4679430..4680671	+	1242	WP_058488666.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12949.77 Da
• Isoelectric Point: 4.6337

>T173391 WP_058488662.1 NZ_CP062122:c4675610-4675263 [Pseudomonas sp. ADPe]
MSKVVIRLTDTAEQSIEDQIHHLAQFQGSQAALQSVLSLLDEIEEKISLTPQGYPISQQASLLGVLNYRELNTGPYRVFY
ELHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIG

Nucleotide

• Length: 348 bp

>T173391 NZ_CP080158:58387-58692 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9500.82 Da
• Isoelectric Point: 8.4753

>AT173391 WP_081884705.1 NZ_CP062122:c4675871-4675620 [Pseudomonas sp. ADPe]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVIESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFD
QPE

Nucleotide

• Length: 252 bp

>AT173391 NZ_CP080158:58167-58385 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A1A5DDR6