Detailed information of TA system TA173373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1240882..1241799
• Replicon: chromosome
• Accession: NZ_CP062074
• Organism: Bacillus velezensis strain BSC16a

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: IGB10_RS06435
• Protein ID: WP_007407256.1
• Coordinates: 1241053..1241799 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: IGB10_RS06430
• Protein ID: WP_003154807.1
• Coordinates: 1240882..1241052 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGB10_RS06395	1236115..1237737	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
IGB10_RS06400	1237750..1238121	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
IGB10_RS06405	1238126..1238323	+	198	WP_032874605.1	XkdX family protein	-
IGB10_RS06410	1238380..1239141	+	762	WP_032874607.1	hypothetical protein	-
IGB10_RS06415	1239193..1239456	+	264	WP_003154815.1	hemolysin XhlA family protein	-
IGB10_RS06420	1239470..1239733	+	264	WP_003154813.1	phage holin	-
IGB10_RS06425	1239747..1240625	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
IGB10_RS06430	1240882..1241052	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IGB10_RS06435	1241053..1241799	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IGB10_RS06440	1241904..1242902	-	999	WP_032874611.1	inorganic phosphate transporter	-
IGB10_RS06445	1242915..1243532	-	618	WP_032874614.1	DUF47 domain-containing protein	-
IGB10_RS06450	1243818..1245134	-	1317	WP_032874616.1	amino acid permease	-
IGB10_RS06455	1245456..1246406	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T173373 WP_007407256.1 NZ_CP062074:c1241799-1241053 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T173373 NZ_CP080155:c2674827-2674651 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT173373 WP_003154807.1 NZ_CP062074:c1241052-1240882 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT173373 NZ_CP080155:c2674605-2674189 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14