Detailed information of TA system TA173369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1363017..1363795
• Replicon: chromosome
• Accession: NZ_CP062066
• Organism: Xanthomonas campestris strain M28

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: IFJ81_RS05715
• Protein ID: WP_011038220.1
• Coordinates: 1363017..1363508 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: IFJ81_RS05720
• Protein ID: WP_011038219.1
• Coordinates: 1363505..1363795 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ81_RS05700	1359345..1360220	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
IFJ81_RS05705	1360870..1361547	+	678	WP_192012502.1	response regulator transcription factor	-
IFJ81_RS05710	1361540..1362874	+	1335	WP_057671653.1	HAMP domain-containing histidine kinase	-
IFJ81_RS05715	1363017..1363508	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
IFJ81_RS05720	1363505..1363795	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
IFJ81_RS05725	1363870..1364271	-	402	WP_040940795.1	type I toxin-antitoxin system SymE family toxin	-
IFJ81_RS05730	1364803..1365426	-	624	WP_011038217.1	dephospho-CoA kinase	-
IFJ81_RS05735	1365440..1366303	-	864	WP_057671659.1	A24 family peptidase	-
IFJ81_RS05740	1366310..1367572	-	1263	WP_057671824.1	type II secretion system F family protein	-
IFJ81_RS05745	1367914..1368342	+	429	WP_057671660.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T173369 WP_011038220.1 NZ_CP062066:c1363508-1363017 [Xanthomonas campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T173369 NZ_CP080155:c2023611-2023237 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT173369 WP_011038219.1 NZ_CP062066:c1363795-1363505 [Xanthomonas campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT173369 NZ_CP080155:c2024068-2023700 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668