Detailed information of TA system TA173361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 766056..766708
• Replicon: chromosome
• Accession: NZ_CP062008
• Organism: Mycolicibacterium mucogenicum DSM 44124

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: A0A8H2JAA1
• Locus tag: C1S78_RS03740
• Protein ID: WP_053854500.1
• Coordinates: 766271..766708 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A8H2JAZ7
• Locus tag: C1S78_RS03735
• Protein ID: WP_020098938.1
• Coordinates: 766056..766268 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1S78_RS03720	761657..762391	+	735	WP_020098935.1	enoyl-CoA hydratase	-
C1S78_RS03725	762391..763506	+	1116	WP_020098936.1	MBL fold metallo-hydrolase	-
C1S78_RS03730	763580..766006	+	2427	WP_171024427.1	HAD-IC family P-type ATPase	-
C1S78_RS03735	766056..766268	+	213	WP_020098938.1	antitoxin	Antitoxin
C1S78_RS03740	766271..766708	+	438	WP_053854500.1	SRPBCC family protein	Toxin
C1S78_RS03745	766851..767621	+	771	WP_020098940.1	VOC family protein	-
C1S78_RS03750	767664..768554	-	891	WP_029121393.1	helix-turn-helix transcriptional regulator	-
C1S78_RS03755	768554..770050	-	1497	WP_053854499.1	carotenoid oxygenase family protein	-
C1S78_RS03760	770047..771246	-	1200	WP_053854498.1	acetyl-CoA acetyltransferase	-
C1S78_RS03765	771355..771615	+	261	WP_053854497.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15632.13 Da
• Isoelectric Point: 9.9623

>T173361 WP_053854500.1 NZ_CP062008:766271-766708 [Mycolicibacterium mucogenicum DSM 44124]
MAKLASSIEVPLSPDKAWEAASDLSRYDDWLSIHRMWRSRLPETLGKGTQISSVVEVKGMLNRVDWTIVHYNPPGSLTLN
GVGKGGVRVKLMAKITPAEAGSNVAFDIHLGGPALFGPIGMVVAAALRSDIHHSLEKFKALFASV

Nucleotide

• Length: 438 bp

>T173361 NZ_CP080150:61137-61289 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7286.06 Da
• Isoelectric Point: 4.2092

>AT173361 WP_020098938.1 NZ_CP062008:766056-766268 [Mycolicibacterium mucogenicum DSM 44124]
MGFLDKVKDLAAQNADKVEAAIEKVGDIVDEKTEGKFKGVVDSAQEAAKKAVAGDQPAAEAPAEGTQEQQ

Nucleotide

• Length: 213 bp

>AT173361 NZ_CP080150:c61085-61025 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT