Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58522..59047 Replicon plasmid unnamed4
Accession NZ_CP061979
Organism Klebsiella pneumoniae strain IR5086

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag H4I67_RS27805 Protein ID WP_013023785.1
Coordinates 58742..59047 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H4I67_RS27800 Protein ID WP_001568025.1
Coordinates 58522..58740 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I67_RS27780 53678..54457 + 780 WP_013023780.1 hypothetical protein -
H4I67_RS27785 54664..56256 + 1593 WP_015344964.1 hypothetical protein -
H4I67_RS27790 56290..57417 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
H4I67_RS27795 57414..57704 + 291 WP_013023783.1 hypothetical protein -
H4I67_RS27800 58522..58740 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H4I67_RS27805 58742..59047 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
H4I67_RS27810 59216..59611 + 396 WP_017899885.1 hypothetical protein -
H4I67_RS27815 59638..59952 + 315 WP_053389906.1 hypothetical protein -
H4I67_RS27820 59963..60979 + 1017 WP_017899884.1 hypothetical protein -
H4I67_RS27825 61177..61971 + 795 WP_004197635.1 site-specific integrase -
H4I67_RS27830 62411..62590 - 180 WP_006788217.1 hypothetical protein -
H4I67_RS27835 62710..63336 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA27 / ARR-3 / aac(6')-Ib-cr - 1..95882 95882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T173358 WP_013023785.1 NZ_CP061979:58742-59047 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T173358 NZ_CP080148:8899-9147 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT173358 WP_001568025.1 NZ_CP061979:58522-58740 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT173358 NZ_CP080148:9144-9491 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References