Detailed information of TA system TA173357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 220625..221295
• Replicon: plasmid unnamed2
• Accession: NZ_CP061977
• Organism: Klebsiella pneumoniae strain IR5086

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: H4I67_RS26755
• Protein ID: WP_004213072.1
• Coordinates: 220852..221295 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: H4I67_RS26750
• Protein ID: WP_004213073.1
• Coordinates: 220625..220855 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I67_RS26725	216848..217747	-	900	WP_004225022.1	nucleotide-binding protein	-
H4I67_RS26730	217737..218027	-	291	WP_004213078.1	hypothetical protein	-
H4I67_RS26735	218379..218585	+	207	WP_004213077.1	hypothetical protein	-
H4I67_RS26740	218575..218868	-	294	WP_004213076.1	hypothetical protein	-
H4I67_RS26745	218884..220017	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
H4I67_RS26750	220625..220855	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H4I67_RS26755	220852..221295	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
H4I67_RS26760	221309..221725	-	417	Protein_218	restriction endonuclease	-
H4I67_RS26765	221729..222022	-	294	Protein_219	transposase	-
H4I67_RS26770	222439..223077	+	639	Protein_220	response regulator	-
H4I67_RS26775	223593..223999	-	407	Protein_221	GAF domain-containing protein	-
H4I67_RS26780	224090..225010	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
H4I67_RS26785	225059..225550	-	492	WP_011251327.1	DM13 domain-containing protein	-
H4I67_RS26790	225613..225888	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA	1..266729	266729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T173357 WP_004213072.1 NZ_CP061977:220852-221295 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T173357 NZ_CP080148:7053-7307 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT173357 WP_004213073.1 NZ_CP061977:220625-220855 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT173357 NZ_CP080148:7304-7546 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620