Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3939621..3940240 Replicon chromosome
Accession NZ_CP061975
Organism Klebsiella pneumoniae strain IR5086

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4I67_RS19295 Protein ID WP_002892050.1
Coordinates 3940022..3940240 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H4I67_RS19290 Protein ID WP_002892066.1
Coordinates 3939621..3939995 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I67_RS19280 3934773..3935966 + 1194 WP_186971451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4I67_RS19285 3935989..3939135 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H4I67_RS19290 3939621..3939995 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H4I67_RS19295 3940022..3940240 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H4I67_RS19300 3940403..3940969 + 567 WP_002892030.1 maltose O-acetyltransferase -
H4I67_RS19305 3940941..3941081 - 141 WP_004147370.1 hypothetical protein -
H4I67_RS19310 3941102..3941572 + 471 WP_020802585.1 YlaC family protein -
H4I67_RS19315 3941547..3942998 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
H4I67_RS19320 3943099..3943797 + 699 WP_023287311.1 GNAT family N-acetyltransferase -
H4I67_RS19325 3943794..3943934 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H4I67_RS19330 3943934..3944197 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173349 WP_002892050.1 NZ_CP061975:3940022-3940240 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T173349 NZ_CP080146:c3274226-3273849 [Escherichia coli]
ATGAAAACATTACCCGACACGCATATACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCGGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGAATTC
AGCCCCTGCACCCGCTCTCAGTTAATCAACAGCATTGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCAG
CCACTACAGAATGGTGAACAACATCACCCTGGGTAAACACTGTGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT173349 WP_002892066.1 NZ_CP061975:3939621-3939995 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173349 NZ_CP080146:c3274673-3274317 [Escherichia coli]
ATCGTGTCAGACACACTCTCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAGGCCCTGTTTTGG
CGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGCATCAGAGGCCGGTTCAGCGATGCGG
ATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCC
CTCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTGTGGTTACGTTTA
TCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References