Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1470079..1470815 Replicon chromosome
Accession NZ_CP061975
Organism Klebsiella pneumoniae strain IR5086

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag H4I67_RS07270 Protein ID WP_032433360.1
Coordinates 1470333..1470815 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H4I67_RS07265 Protein ID WP_003026799.1
Coordinates 1470079..1470345 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I67_RS07240 1465725..1466864 + 1140 WP_032433368.1 mannitol dehydrogenase -
H4I67_RS07245 1466893..1467555 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
H4I67_RS07250 1467539..1468543 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
H4I67_RS07255 1468561..1469193 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
H4I67_RS07260 1469203..1469766 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
H4I67_RS07265 1470079..1470345 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H4I67_RS07270 1470333..1470815 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
H4I67_RS07275 1471015..1472418 + 1404 WP_187982397.1 ISNCY-like element ISKpn21 family transposase -
H4I67_RS07280 1472447..1473079 - 633 WP_001567369.1 hypothetical protein -
H4I67_RS07285 1473459..1474058 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
H4I67_RS07290 1474271..1475215 - 945 WP_077254249.1 fimbrial protein -
H4I67_RS07295 1475227..1475805 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1438024..1485107 47083
- flank IS/Tn - - 1471015..1472418 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T173339 WP_032433360.1 NZ_CP061975:1470333-1470815 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T173339 NZ_CP080146:977347-977601 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT173339 WP_003026799.1 NZ_CP061975:1470079-1470345 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT173339 NZ_CP080146:977099-977350 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References