Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1453388..1454031 Replicon chromosome
Accession NZ_CP061975
Organism Klebsiella pneumoniae strain IR5086

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag H4I67_RS07175 Protein ID WP_032433387.1
Coordinates 1453615..1454031 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H4I67_RS07170 Protein ID WP_001261276.1
Coordinates 1453388..1453618 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I67_RS07155 1448410..1449497 + 1088 Protein_1396 transcriptional regulator -
H4I67_RS07160 1449500..1451740 + 2241 WP_045326788.1 NTPase -
H4I67_RS07165 1452268..1453083 - 816 WP_032433388.1 hypothetical protein -
H4I67_RS07170 1453388..1453618 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H4I67_RS07175 1453615..1454031 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I67_RS07180 1454187..1455167 + 981 WP_032433385.1 hypothetical protein -
H4I67_RS07185 1455362..1456933 - 1572 WP_032433383.1 ATP-binding protein -
H4I67_RS07190 1457252..1457500 + 249 WP_032433382.1 hypothetical protein -
H4I67_RS07195 1457559..1458077 + 519 WP_045326794.1 hypothetical protein -
H4I67_RS07200 1458108..1458599 + 492 WP_032433378.1 hypothetical protein -
H4I67_RS07205 1458659..1458862 + 204 WP_032433376.1 hemolysin expression modulator Hha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1438024..1485107 47083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T173338 WP_032433387.1 NZ_CP061975:1453615-1454031 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T173338 NZ_CP080146:977099-977350 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT173338 WP_001261276.1 NZ_CP061975:1453388-1453618 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT173338 NZ_CP080146:977347-977601 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References