Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56491..57016 Replicon plasmid unnamed2
Accession NZ_CP061972
Organism Klebsiella pneumoniae strain IR5755

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag H4I70_RS27550 Protein ID WP_013023785.1
Coordinates 56711..57016 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H4I70_RS27545 Protein ID WP_001568025.1
Coordinates 56491..56709 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I70_RS27525 51647..52426 + 780 WP_013023780.1 hypothetical protein -
H4I70_RS27530 52633..54225 + 1593 WP_015344964.1 hypothetical protein -
H4I70_RS27535 54259..55386 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
H4I70_RS27540 55383..55673 + 291 WP_013023783.1 hypothetical protein -
H4I70_RS27545 56491..56709 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H4I70_RS27550 56711..57016 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
H4I70_RS27555 57185..57580 + 396 WP_017899885.1 hypothetical protein -
H4I70_RS27560 57607..57921 + 315 WP_053389906.1 hypothetical protein -
H4I70_RS27565 57932..58948 + 1017 WP_017899884.1 hypothetical protein -
H4I70_RS27570 59146..59940 + 795 WP_004197635.1 site-specific integrase -
H4I70_RS27575 60412..60591 - 180 WP_006788217.1 hypothetical protein -
H4I70_RS27580 60711..61337 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrS1 / blaCTX-M-3 / blaTEM-1B / floR / tet(A) / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 - 1..121732 121732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T173334 WP_013023785.1 NZ_CP061972:56711-57016 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T173334 NZ_CP080143:c29176-28994 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT173334 WP_001568025.1 NZ_CP061972:56491-56709 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT173334 NZ_CP080143:c28969-28532 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAGGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTAG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAAAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAGCTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References