Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5369738..5370363 Replicon chromosome
Accession NZ_CP061970
Organism Klebsiella pneumoniae strain IR5755

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H4I70_RS26315 Protein ID WP_002882817.1
Coordinates 5369738..5370121 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H4I70_RS26320 Protein ID WP_004150355.1
Coordinates 5370121..5370363 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I70_RS26300 5367104..5368006 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H4I70_RS26305 5368003..5368638 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H4I70_RS26310 5368635..5369564 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H4I70_RS26315 5369738..5370121 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I70_RS26320 5370121..5370363 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H4I70_RS26325 5370568..5371485 + 918 WP_009484979.1 alpha/beta hydrolase -
H4I70_RS26330 5371499..5372440 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H4I70_RS26335 5372485..5372922 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H4I70_RS26340 5372919..5373779 - 861 WP_023280688.1 virulence factor BrkB family protein -
H4I70_RS26345 5373773..5374372 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T173333 WP_002882817.1 NZ_CP061970:c5370121-5369738 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T173333 NZ_CP080142:43155-43442 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT173333 WP_004150355.1 NZ_CP061970:c5370363-5370121 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT173333 NZ_CP080142:42907-43158 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References