Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4153369..4153988 Replicon chromosome
Accession NZ_CP061970
Organism Klebsiella pneumoniae strain IR5755

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4I70_RS20530 Protein ID WP_002892050.1
Coordinates 4153770..4153988 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H4I70_RS20525 Protein ID WP_002892066.1
Coordinates 4153369..4153743 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I70_RS20515 4148521..4149714 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4I70_RS20520 4149737..4152883 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H4I70_RS20525 4153369..4153743 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H4I70_RS20530 4153770..4153988 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H4I70_RS20535 4154147..4154713 + 567 WP_002892030.1 maltose O-acetyltransferase -
H4I70_RS20540 4154685..4154825 - 141 WP_004147370.1 hypothetical protein -
H4I70_RS20545 4154846..4155316 + 471 WP_002892026.1 YlaC family protein -
H4I70_RS20550 4155291..4156742 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
H4I70_RS20555 4156843..4157541 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
H4I70_RS20560 4157538..4157678 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H4I70_RS20565 4157678..4157941 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173330 WP_002892050.1 NZ_CP061970:4153770-4153988 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T173330 NZ_CP080141:7053-7307 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT173330 WP_002892066.1 NZ_CP061970:4153369-4153743 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173330 NZ_CP080141:7304-7546 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References