Detailed information of TA system TA173329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2709244..2710832
• Replicon: chromosome
• Accession: NZ_CP061970
• Organism: Klebsiella pneumoniae strain IR5755

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: J2LTC9
• Locus tag: H4I70_RS13245
• Protein ID: WP_004148372.1
• Coordinates: 2709244..2710566 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H4I70_RS13250
• Protein ID: WP_004176198.1
• Coordinates: 2710566..2710832 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I70_RS13230	2704300..2706801	+	2502	WP_040025883.1	fimbrial biogenesis outer membrane usher protein	-
H4I70_RS13235	2706789..2707793	+	1005	WP_040025882.1	type 1 fimbrial protein	-
H4I70_RS13240	2707935..2709227	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
H4I70_RS13245	2709244..2710566	-	1323	WP_004148372.1	type II toxin-antitoxin system HipA family toxin	Toxin
H4I70_RS13250	2710566..2710832	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H4I70_RS13255	2711218..2711544	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
H4I70_RS13260	2711541..2712041	-	501	WP_020804988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
H4I70_RS13265	2712019..2713398	-	1380	WP_004199850.1	purine permease	-
H4I70_RS13270	2713744..2714898	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
H4I70_RS13275	2714879..2715802	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2506534..2746435	239901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49127.45 Da
• Isoelectric Point: 7.5357

>T173329 WP_004148372.1 NZ_CP061970:c2710566-2709244 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSLRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T173329 NZ_CP080139:c5395584-5395366 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT173329 WP_004176198.1 NZ_CP061970:c2710832-2710566 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT173329 NZ_CP080139:c5395984-5395610 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: J2LTC9