Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1538484..1539127 Replicon chromosome
Accession NZ_CP061970
Organism Klebsiella pneumoniae strain IR5755

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag H4I70_RS07550 Protein ID WP_032433387.1
Coordinates 1538711..1539127 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H4I70_RS07545 Protein ID WP_001261276.1
Coordinates 1538484..1538714 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I70_RS07530 1533506..1534593 + 1088 Protein_1472 transcriptional regulator -
H4I70_RS07535 1534596..1536836 + 2241 WP_045326788.1 NTPase -
H4I70_RS07540 1537364..1538179 - 816 WP_040025605.1 hypothetical protein -
H4I70_RS07545 1538484..1538714 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H4I70_RS07550 1538711..1539127 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I70_RS07555 1539283..1540263 + 981 WP_032433385.1 hypothetical protein -
H4I70_RS07560 1540458..1542029 - 1572 WP_040025608.1 ATP-binding protein -
H4I70_RS07565 1542348..1542596 + 249 WP_032433382.1 hypothetical protein -
H4I70_RS07570 1542655..1543173 + 519 WP_052445790.1 hypothetical protein -
H4I70_RS07575 1543204..1543695 + 492 WP_040025612.1 hypothetical protein -
H4I70_RS07580 1543755..1543958 + 204 WP_032433376.1 hemolysin expression modulator Hha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1524038..1566855 42817


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T173319 WP_032433387.1 NZ_CP061970:1538711-1539127 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T173319 NZ_CP080139:c3664686-3664579 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT173319 WP_001261276.1 NZ_CP061970:1538484-1538714 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT173319 NZ_CP080139:3664734-3664800 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References