Detailed information of TA system TA173317

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 741385..742220
• Replicon: chromosome
• Accession: NZ_CP061970
• Organism: Klebsiella pneumoniae strain IR5755

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1V3VXN0
• Locus tag: H4I70_RS03645
• Protein ID: WP_000854801.1
• Coordinates: 741843..742220 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1V3VXQ4
• Locus tag: H4I70_RS03640
• Protein ID: WP_001360188.1
• Coordinates: 741385..741753 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I70_RS03615	738500..739318	+	819	WP_001234702.1	DUF945 domain-containing protein	-
H4I70_RS03620	739409..739894	+	486	WP_000214418.1	antirestriction protein	-
H4I70_RS03625	739910..740386	+	477	WP_001186725.1	RadC family protein	-
H4I70_RS03630	740455..740676	+	222	WP_000692334.1	DUF987 domain-containing protein	-
H4I70_RS03635	740691..741335	+	645	WP_000086744.1	hypothetical protein	-
H4I70_RS03640	741385..741753	+	369	WP_001360188.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H4I70_RS03645	741843..742220	+	378	WP_000854801.1	type IV toxin-antitoxin system toxin CbtA	Toxin
H4I70_RS03650	742217..742705	+	489	WP_000761658.1	hypothetical protein	-
H4I70_RS03655	742717..742914	+	198	WP_000839269.1	DUF957 domain-containing protein	-
H4I70_RS03660	742999..743844	+	846	Protein_721	DUF4942 domain-containing protein	-
H4I70_RS03670	744144..744650	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
H4I70_RS03675	744750..746591	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	blaCTX-M-65 / fosA3 / floR / aph(4)-Ia / aac(3)-IVa / blaTEM-1B	-	701663..748555	46892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13881.87 Da
• Isoelectric Point: 7.3219

>T173317 WP_000854801.1 NZ_CP061970:741843-742220 [Klebsiella pneumoniae]
MKTLPVLPGQAASSRPSPVEIWQILLSQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T173317 NZ_CP080139:c3194387-3194073 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.27 Da
• Isoelectric Point: 6.2044

>AT173317 WP_001360188.1 NZ_CP061970:741385-741753 [Klebsiella pneumoniae]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT173317 NZ_CP080139:c3194076-3193660 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A1V3VXN0

Antitoxin

• AlphaFold DB: A0A1V3VXQ4