Detailed information of TA system TA173315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 128594..129237
• Replicon: plasmid unnamed1
• Accession: NZ_CP061966
• Organism: Klebsiella pneumoniae strain LZK001

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H4I72_RS26165
• Protein ID: WP_016236302.1
• Coordinates: 128594..129010 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: H4I72_RS26170
• Protein ID: WP_001261282.1
• Coordinates: 129007..129237 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I72_RS26145	123962..124312	+	351	WP_004187110.1	DUF305 domain-containing protein	-
H4I72_RS26150	124498..125412	-	915	WP_153378766.1	HNH endonuclease	-
H4I72_RS26155	125951..126973	-	1023	WP_032439672.1	helicase UvrD	-
H4I72_RS26160	126958..128520	-	1563	WP_004206609.1	AAA family ATPase	-
H4I72_RS26165	128594..129010	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
H4I72_RS26170	129007..129237	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H4I72_RS26175	129194..129616	+	423	WP_046624301.1	hypothetical protein	-
H4I72_RS26180	130157..130588	+	432	WP_174805835.1	hypothetical protein	-
H4I72_RS26185	131206..132660	-	1455	WP_072001611.1	EAL domain-containing protein	-
H4I72_RS26190	133264..133521	+	258	WP_023292103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..150870	150870

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T173315 WP_016236302.1 NZ_CP061966:c129010-128594 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T173315 NZ_CP080139:3052821-3053198 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGCCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATACGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT173315 WP_001261282.1 NZ_CP061966:c129237-129007 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT173315 NZ_CP080139:3052363-3052731 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3