Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5200056..5200681 Replicon chromosome
Accession NZ_CP061965
Organism Klebsiella pneumoniae strain LZK001

Toxin (Protein)


Gene name vapC Uniprot ID A0A483ITU7
Locus tag H4I72_RS25040 Protein ID WP_048334590.1
Coordinates 5200056..5200439 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H4I72_RS25045 Protein ID WP_004150355.1
Coordinates 5200439..5200681 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I72_RS25025 5197422..5198324 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H4I72_RS25030 5198321..5198956 + 636 WP_076027700.1 formate dehydrogenase cytochrome b556 subunit -
H4I72_RS25035 5198953..5199882 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H4I72_RS25040 5200056..5200439 - 384 WP_048334590.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I72_RS25045 5200439..5200681 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H4I72_RS25050 5200886..5201803 + 918 WP_004181612.1 alpha/beta hydrolase -
H4I72_RS25055 5201817..5202758 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H4I72_RS25060 5202803..5203240 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H4I72_RS25065 5203237..5204097 - 861 WP_023280688.1 virulence factor BrkB family protein -
H4I72_RS25070 5204091..5204690 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14381.60 Da        Isoelectric Point: 7.3178

>T173314 WP_048334590.1 NZ_CP061965:c5200439-5200056 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGITGVVTPYEIHPE

Download         Length: 384 bp

>T173314 NZ_CP080139:c2938030-2937623 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT173314 WP_004150355.1 NZ_CP061965:c5200681-5200439 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT173314 NZ_CP080139:c2938277-2938011 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483ITU7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References