Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1062215..1062834 Replicon chromosome
Accession NZ_CP061965
Organism Klebsiella pneumoniae strain LZK001

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4I72_RS05155 Protein ID WP_002892050.1
Coordinates 1062215..1062433 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H4I72_RS05160 Protein ID WP_002892066.1
Coordinates 1062460..1062834 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I72_RS05120 1058262..1058525 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
H4I72_RS05125 1058525..1058665 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H4I72_RS05130 1058662..1059360 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
H4I72_RS05135 1059461..1060912 + 1452 WP_076027561.1 PLP-dependent aminotransferase family protein -
H4I72_RS05140 1060887..1061357 - 471 WP_002892026.1 YlaC family protein -
H4I72_RS05145 1061378..1061518 + 141 WP_004147370.1 hypothetical protein -
H4I72_RS05150 1061490..1062056 - 567 WP_002892030.1 maltose O-acetyltransferase -
H4I72_RS05155 1062215..1062433 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H4I72_RS05160 1062460..1062834 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H4I72_RS05165 1063319..1066465 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H4I72_RS05170 1066488..1067681 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173297 WP_002892050.1 NZ_CP061965:c1062433-1062215 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T173297 NZ_CP080133:c3629992-3629594 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT173297 WP_002892066.1 NZ_CP061965:c1062834-1062460 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173297 NZ_CP080133:c3630288-3629995 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References