Detailed information of TA system TA173292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 61235..61905
• Replicon: plasmid unnamed1
• Accession: NZ_CP061962
• Organism: Klebsiella pneumoniae strain JNK002

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: H4I73_RS27160
• Protein ID: WP_004213072.1
• Coordinates: 61462..61905 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: H4I73_RS27155
• Protein ID: WP_004213073.1
• Coordinates: 61235..61465 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I73_RS27130	57458..58357	-	900	WP_004225022.1	nucleotide-binding protein	-
H4I73_RS27135	58347..58637	-	291	WP_004213078.1	hypothetical protein	-
H4I73_RS27140	58989..59195	+	207	WP_004213077.1	hypothetical protein	-
H4I73_RS27145	59185..59478	-	294	WP_004213076.1	hypothetical protein	-
H4I73_RS27150	59494..60627	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
H4I73_RS27155	61235..61465	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H4I73_RS27160	61462..61905	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
H4I73_RS27165	61919..62335	-	417	Protein_65	restriction endonuclease	-
H4I73_RS27170	62339..62632	-	294	Protein_66	transposase	-
H4I73_RS27175	63049..63701	+	653	Protein_67	response regulator	-
H4I73_RS27180	64216..64622	-	407	Protein_68	GAF domain-containing protein	-
H4I73_RS27185	64713..65633	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
H4I73_RS27190	65682..66173	-	492	WP_011251327.1	DM13 domain-containing protein	-
H4I73_RS27195	66236..66511	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / ant(2'')-Ia / blaSHV-2 / catA1	rmpA / iutA / iucD / iucC / iucB / iucA	1..307252	307252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T173292 WP_004213072.1 NZ_CP061962:61462-61905 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T173292 NZ_CP080133:1993660-1993763 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT173292 WP_004213073.1 NZ_CP061962:61235-61465 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT173292 NZ_CP080133:c1993801-1993530 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620