Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4750463..4751154 Replicon chromosome
Accession NZ_CP061961
Organism Klebsiella pneumoniae strain JNK002

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag H4I73_RS23620 Protein ID WP_025987721.1
Coordinates 4750463..4750804 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag H4I73_RS23625 Protein ID WP_019725272.1
Coordinates 4750828..4751154 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I73_RS23605 4747429..4748664 - 1236 WP_124044824.1 hypothetical protein -
H4I73_RS23610 4749117..4749428 - 312 WP_073568733.1 hypothetical protein -
H4I73_RS23615 4749515..4750348 - 834 WP_025987720.1 DUF4942 domain-containing protein -
H4I73_RS23620 4750463..4750804 - 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
H4I73_RS23625 4750828..4751154 - 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
H4I73_RS23630 4751168..4751644 - 477 WP_019725273.1 DNA repair protein RadC -
H4I73_RS23635 4751654..4752100 - 447 WP_025987722.1 antirestriction protein -
H4I73_RS23640 4752312..4753001 - 690 WP_009309812.1 hypothetical protein -
H4I73_RS23645 4753566..4754456 - 891 WP_019725276.1 50S ribosome-binding GTPase -
H4I73_RS23650 4754539..4755627 - 1089 WP_019725277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4733388..4773766 40378


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T173288 WP_025987721.1 NZ_CP061961:c4750804-4750463 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

>T173288 NZ_CP080133:919759-920166 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT173288 WP_019725272.1 NZ_CP061961:c4751154-4750828 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp

>AT173288 NZ_CP080133:919512-919778 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References