Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4089043..4089662 Replicon chromosome
Accession NZ_CP061961
Organism Klebsiella pneumoniae strain JNK002

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4I73_RS20435 Protein ID WP_002892050.1
Coordinates 4089444..4089662 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H4I73_RS20430 Protein ID WP_002892066.1
Coordinates 4089043..4089417 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I73_RS20420 4084195..4085388 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4I73_RS20425 4085411..4088557 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H4I73_RS20430 4089043..4089417 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H4I73_RS20435 4089444..4089662 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H4I73_RS20440 4089821..4090387 + 567 WP_002892030.1 maltose O-acetyltransferase -
H4I73_RS20445 4090359..4090499 - 141 WP_004147370.1 hypothetical protein -
H4I73_RS20450 4090520..4090990 + 471 WP_002892026.1 YlaC family protein -
H4I73_RS20455 4090965..4092416 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
H4I73_RS20460 4092517..4093215 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
H4I73_RS20465 4093212..4093352 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H4I73_RS20470 4093352..4093615 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173286 WP_002892050.1 NZ_CP061961:4089444-4089662 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T173286 NZ_CP080133:714805-715101 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT173286 WP_002892066.1 NZ_CP061961:4089043-4089417 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173286 NZ_CP080133:715103-715498 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References