Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9375..10018 Replicon plasmid unnamed3
Accession NZ_CP061960
Organism Klebsiella pneumoniae strain IR5726

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H4I74_RS27885 Protein ID WP_001044770.1
Coordinates 9375..9791 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H4I74_RS27890 Protein ID WP_001261282.1
Coordinates 9788..10018 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I74_RS27865 6207..6431 - 225 WP_049090873.1 helix-turn-helix domain-containing protein -
H4I74_RS27870 6613..7176 + 564 WP_048981108.1 transcriptional regulator -
H4I74_RS27875 7384..8073 + 690 WP_206971191.1 IS6 family transposase -
H4I74_RS27880 8123..9301 - 1179 Protein_13 AAA family ATPase -
H4I74_RS27885 9375..9791 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I74_RS27890 9788..10018 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H4I74_RS27895 9975..10436 + 462 WP_014343465.1 hypothetical protein -
H4I74_RS27900 10597..11541 + 945 WP_011977810.1 hypothetical protein -
H4I74_RS27905 11578..11970 + 393 WP_011977811.1 hypothetical protein -
H4I74_RS27910 12028..12549 + 522 WP_013214008.1 hypothetical protein -
H4I74_RS27915 12595..12798 + 204 WP_011977813.1 hypothetical protein -
H4I74_RS27920 12828..13832 + 1005 WP_011977814.1 hypothetical protein -
H4I74_RS27925 14016..14795 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / blaKPC-2 / blaSHV-12 - 1..144349 144349
- flank IS/Tn - - 7333..8073 740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T173265 WP_001044770.1 NZ_CP061960:c9791-9375 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T173265 NZ_CP080126:1976839-1976942 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT173265 WP_001261282.1 NZ_CP061960:c10018-9788 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT173265 NZ_CP080126:c1976980-1976709 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References