Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5360147..5360772 Replicon chromosome
Accession NZ_CP061957
Organism Klebsiella pneumoniae strain IR5726

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H4I74_RS26290 Protein ID WP_002882817.1
Coordinates 5360147..5360530 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H4I74_RS26295 Protein ID WP_004150355.1
Coordinates 5360530..5360772 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I74_RS26275 5357513..5358415 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H4I74_RS26280 5358412..5359047 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H4I74_RS26285 5359044..5359973 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H4I74_RS26290 5360147..5360530 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I74_RS26295 5360530..5360772 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H4I74_RS26300 5360977..5361894 + 918 WP_002882812.1 alpha/beta hydrolase -
H4I74_RS26305 5361908..5362849 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H4I74_RS26310 5362894..5363331 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H4I74_RS26315 5363328..5364188 - 861 WP_002882807.1 virulence factor BrkB family protein -
H4I74_RS26320 5364182..5364781 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T173264 WP_002882817.1 NZ_CP061957:c5360530-5360147 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T173264 NZ_CP080126:1792973-1793227 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT173264 WP_004150355.1 NZ_CP061957:c5360772-5360530 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT173264 NZ_CP080126:1792725-1792976 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References