Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2744551..2746139 Replicon chromosome
Accession NZ_CP061957
Organism Klebsiella pneumoniae strain IR5726

Toxin (Protein)


Gene name hipA Uniprot ID A0A0H3GN97
Locus tag H4I74_RS13545 Protein ID WP_004199851.1
Coordinates 2744551..2745873 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag H4I74_RS13550 Protein ID WP_002904790.1
Coordinates 2745873..2746139 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I74_RS13530 2739616..2742117 + 2502 WP_004199853.1 fimbrial biogenesis outer membrane usher protein -
H4I74_RS13535 2742105..2743109 + 1005 WP_004199852.1 type 1 fimbrial protein -
H4I74_RS13540 2743242..2744534 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
H4I74_RS13545 2744551..2745873 - 1323 WP_004199851.1 type II toxin-antitoxin system HipA family toxin Toxin
H4I74_RS13550 2745873..2746139 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
H4I74_RS13555 2746405..2746731 - 327 WP_002904788.1 hydroxyisourate hydrolase -
H4I74_RS13560 2746728..2747228 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
H4I74_RS13565 2747206..2748585 - 1380 WP_004199850.1 purine permease -
H4I74_RS13570 2748931..2750085 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
H4I74_RS13575 2750066..2750989 - 924 WP_004199849.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49128.44 Da        Isoelectric Point: 7.2487

>T173259 WP_004199851.1 NZ_CP061957:c2745873-2744551 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T173259 NZ_CP080126:848564-848971 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT173259 WP_002904790.1 NZ_CP061957:c2746139-2745873 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT173259 NZ_CP080126:848317-848583 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GN97

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References