Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5281283..5281908 Replicon chromosome
Accession NZ_CP061952
Organism Klebsiella pneumoniae strain IR5654

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H4I75_RS25945 Protein ID WP_002882817.1
Coordinates 5281283..5281666 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H4I75_RS25950 Protein ID WP_004150355.1
Coordinates 5281666..5281908 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I75_RS25930 5278649..5279551 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H4I75_RS25935 5279548..5280183 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H4I75_RS25940 5280180..5281109 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H4I75_RS25945 5281283..5281666 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I75_RS25950 5281666..5281908 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H4I75_RS25955 5282113..5283030 + 918 WP_002882812.1 alpha/beta hydrolase -
H4I75_RS25960 5283044..5283985 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H4I75_RS25965 5284030..5284467 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H4I75_RS25970 5284464..5285324 - 861 WP_002882807.1 virulence factor BrkB family protein -
H4I75_RS25975 5285318..5285917 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T173244 WP_002882817.1 NZ_CP061952:c5281666-5281283 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T173244 NZ_CP080121:1899710-1899813 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT173244 WP_004150355.1 NZ_CP061952:c5281908-5281666 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT173244 NZ_CP080121:c1899851-1899580 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References