Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4083897..4084516 Replicon chromosome
Accession NZ_CP061952
Organism Klebsiella pneumoniae strain IR5654

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4I75_RS20200 Protein ID WP_002892050.1
Coordinates 4084298..4084516 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H4I75_RS20195 Protein ID WP_002892066.1
Coordinates 4083897..4084271 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I75_RS20185 4079049..4080242 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4I75_RS20190 4080265..4083411 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H4I75_RS20195 4083897..4084271 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H4I75_RS20200 4084298..4084516 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H4I75_RS20205 4084675..4085241 + 567 WP_002892030.1 maltose O-acetyltransferase -
H4I75_RS20210 4085213..4085353 - 141 WP_004147370.1 hypothetical protein -
H4I75_RS20215 4085374..4085844 + 471 WP_002892026.1 YlaC family protein -
H4I75_RS20220 4085819..4087270 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
H4I75_RS20225 4087371..4088069 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
H4I75_RS20230 4088066..4088206 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H4I75_RS20235 4088206..4088469 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173240 WP_002892050.1 NZ_CP061952:4084298-4084516 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T173240 NZ_CP080121:825419-825826 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT173240 WP_002892066.1 NZ_CP061952:4083897-4084271 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173240 NZ_CP080121:825172-825438 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References